By Robert A. Andersen
Algal Culturing Techniques is a accomplished reference on all points of the isolation and cultivation of marine and freshwater algae, together with seaweeds. it's divided into seven components that hide background, media coaching, isolation and purification ideas, mass culturing options, telephone counting and progress dimension suggestions, and reports on subject matters and functions of algal tradition recommendations for environmental investigations.
Algal Culturing Techniques was once constructed to function either a brand new textbook and key reference for phycologists and others learning aquatic platforms, aquaculture and environmental sciences. scholars of algal ecology, marine botany, marine phycology, and microbial ecology will benefit from the hands-on technique for culturing quite a few algae from clean and marine waters. Researchers in undefined, resembling aquaculture, pharmaceutical, foodstuffs, and biotechnology businesses will locate an authoritative and finished reference.
- Sponsored via the Phycological Society of America
- Features colour photos and illustrations throughout
- Describes culturing tools starting from the try tube to open air ponds and coastal seaweed farms
- Details isolation options starting from conventional micropipette to automatic circulate cytometeric methods
- Includes purification, progress, upkeep, and cryopreservation techniques
- Highlights tools for estimating algal populations, progress premiums, setting apart and measuring algal pigments, and detecting and culturing algal viruses
- Features a entire appendix of approximately 50 algal tradition medium recipes
- Includes a word list of phycological terms
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Additional resources for Algal Culturing Techniques
1. Natural Seawater The general steps are as follows: 1. Obtain good–quality NW with salinity >30, if possible. 2. Filter as soon as possible, and store the seawater in the dark and cold (4°C). 3. 2 mm), autoclaving, ultraviolet light treatment, or pasteurization. 4. Add macronutrients, Fe-EDTA, trace metals, and vitamins aseptically after autoclaving. Mix thoroughly after the addition of each stock. 2. Artificial Seawater Artiﬁcial or synthetic seawater consists of two parts: the basal (main) salts that form the “basal seawater” and the enrichment solution (often the same as the enrichment solution that is added to NW).
Preoccupation with making a complete medium autoclavable without precipitation led to the following extensive modiﬁcations in recipes: 1. Addition of synthetic metal chelators such as EDTA or nitrilotriacetic acid (NTA) to decrease metal precipitates 2. 5 range), because the amount of precipitate increased as the pH rose during autoclaving 3. Reduction in salinity, thereby reducing the amount of salts available for precipitation 4. Replacement of Mg2+ and Ca2+ with more soluble univalent salts 5.
Commercial preparations are synthetic mixes and can be purchased in various amounts. One of the best is Instant Ocean, produced by Aquarium Systems, Inc. (King and Spotte 1974). The ion ratio of the major salts Marine Culture Media 29 is very close to NW. Macronutrients, trace metals, and vitamins must be added. If large-scale preparation is required, this may be an economical alternative to buying individual salts and weighing and mixing the salts. For all the AW basal salt recipes, if contamination is a concern, then we recommend that you analyze the basal medium for copper, zinc, cobalt, lead, manganese, nickel, chromium, etc.